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Charlotte of Albret (1480 – 11 March 1514), Dame de Châlus, was a wealthy French noblewoman of the Albret family. She was the sister of King John III of Navarre and the wife of the widely notorious Cesare Borgia, whom she married in 1499. She was the mother of his only legitimate child, Louise Borgia, to whom she acted as regent of the Duchy of Valentinois from 1507 to 1514, following the death of Cesare.